First NameMadiha
Last NameBasit
Emailmadiha73basit@gmail.com
Supervisor NameDr Abu Baker Siddique
UniversityGovt College University Faisalabad
CountryPakistan
KeywordsBurn wounds, Viro Care GZ-08™, Antibacterial activity, Antifungal activity, Disc diffusion method, Broth dilution method, Phenol coefficient assay, Minimum Inhibitory Concentration
Publication DateJune 6, 2015
DegreeMasters
DomainBiology

In-vitro determination of Antibacterial and Antifungal activity of Viro Care GZ-08™ against Burn Wound Pathogens 2014

Abstract

Burn is a type of injury to the skin caused by heat, electricity or chemicals. Keeping in view the importance of burn wound infections and resistant strains of pathogen, the present study has been designed to isolate and identify the pathogens from burn wounds and evaluate the in-vitro antibacterial, antifungal and disinfectant activity of a Japanese product Viro Care GZ-08™ (oral, spray and ointment) against these pathogens. Samples were collected from burn patients that have first, second, third and fourth week of hospitalization. Pathogens were isolated on differential and selective media. Bacterial isolates were identified through morphology and biochemical test and fungal isolates by microscopic and macroscopic characteristic. Disc diffusion assay was done using Muller Hinton agar medium and Minimum Inhibitory Concentration (MIC) was determined using broth dilution method against isolated pathogens. The disinfectant activity of GZ-08™ spray was tested using Phenol coefficient assay. 91% samples were positive for bacterial and fungal infections. Common isolated pathogens were Staphylococcus aureus (27%), followed by Bacillus subtilis (20%), Escherichia coli (18%), Candida albicans (15%), Aspergillus flavus (11%) and Salmonella typhi (9%). In-vitro study showed that GZ-08™ (oral, spry and ointment) has antibacterial activity against S. aureus (12.9±1.4, 12.8±1.6 and 12.5±1.6) mm and S. typhi (8.4±1, 8.5±1 and 8.6±0.9) mm while B. subtilis, E. coli, C. albicans and A. flavus were resistant with disc diffusion assay. GZ-08™ has MIC against S. aureus (20.8±6, 20±6.34 and 21.7±9.7) μl and S. typhi (35±13.7, 40±13.7 and 40±13.7) μl while other species were resistant. There was no disinfectant activity of GZ-08™ spray against all isolates. Statistical analysis with one way ANOVA showed that statistical differences (P0.05) were found in antibacterial activity of oral, spray and ointment of GZ-08™ against isolates. In-vitro study showed that GZ-08™ is effective against S. aureus and S. typhi while it cannot be used against E. coli, B. subtilis, C. albicans and A. flavus. GZ-08™ cannot be used as disinfectant against all bacterial and fungal isolates.

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